Composite

Part:BBa_K3440013:Design

Designed by: Julie Cordier   Group: iGEM20_Stockholm   (2020-10-23)


LuxR-Myc under constitutive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 874
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed to show that LuxR can be expressed under Pconst. A myc-tag was added to monitor LuxR expression. A moderate strength RBS was used.

Source

luxR from Vibrio fischeri

References